Mister Rogers mentions that he has never been able to whistle and shares a collection of whistles he has brought with him. After he demonstrates the various styles of whistles, Mister Rogers sings What Would You Like To Do Today.
Mr. McFeely stops by with a slide whistle. Mister Rogers invites him inside and plays the whistle while Mr. McFeely sings One and One are Two.
In the Neighborhood of Make-Believe, Mr. Anybody is a whistler. He stops by the clock where Daniel Striped Tiger asks him to demonstrate marching whistling and sleeping whistling. Daniel remembers being whistled to sleep by his mother when he was younger. Mr. Anybody sings I Like You As You Are before trying out several kinds of whistles with Daniel.
Stopping by the Museum-Go-Round, Mr. Anybody asks Mrs. Frogg if she has an extra whistle that he might give tot he Trolley. She is able to provide him with a trolley whistle as well as a kerchief to clean the whistles between uses. At the tree, X the Owl and Henrietta Pussycat take turns trying the trolley whistle before Mr. Anybody gives it to the Neighborhood Trolley.
Back at the house, Mister Rogers cleans all of the whistles he brought with him as he sings You Are Special.
For details, notes, and more screenshots from Episode 0101 of Mister Rogers' Neighborhood, visit the Neighborhood Archive.